JCPM2025.12.09 | Female Sexual Dysfunction Prevalence | PRP Antibacterial Properties | Schwann Cells


JCPM2025.12.09

The following is an edited transcript of theย Journal Club with Pearls & Marketingย (JCPM) of December 9, 2025, with Charles Runels, MD. ย 

>-> The PDF transcript of this live journal club can be seen here <-<

Topics Covered

  • Antibacterial properties of platelet-rich plasma
  • PRP and chronic, hard-to-treat infections
  • Technique, sterility, and contamination risk
  • Importance of FDA-approved PRP preparation systems
  • Neurogenic and Schwann-cell effects of PRP
  • Aging, trauma, and loss of genital nerve density
  • Caution regarding exosomes and regulatory exposure
  • Clinical positioning and communication with patients
  • New Directory Functionality, Profiles, and Conversion Mechanics
  • Review Articles, Visual Education, and Adjunctive Therapies
  • Q&A on female sexual dysfunctionย prevalence
  • AI tool demonstration

Charles Runels, MD
Author, researcher, and inventor of the Vampire Faceliftยฎ, Orchid Shotยฎ (O-Shotยฎ), Priapus Shotยฎ (P-Shotยฎ), Priapus Toxinยฎ, Vampire Breast Liftยฎ, and Vampire Wing Liftยฎ, & Clitoxinยฎ procedures.

Transcript

Welcome to our Journal Club. I think weโ€™ll finish in about 30 minutes tonight. We have four or five beautiful papers that should encourage us in what we do, a couple of tips to be garnered from them. And I wrote two emails for you that will help you, hopefully, find people who need what you know how to do.

So letโ€™s start with the research.

Platelet-Rich Plasma: Antibacterial Properties, Technique Integrity, Neurogenesis, and Clinical Translation

Antibacterial Properties of Platelet-Rich Plasma and Clinical Observations

This one, regarding antibacterial properties, is worth revisiting (the research idea is not new).[1]ย Weโ€™ve had research, dating back over a decade, suggesting that platelet-rich plasma can help with methicillin-resistantย Staph aureusย and treat hard-to-heal wound infections.[2]ย [3]ย [4]

I bring this up because we, anecdotally, routinely hear about people receiving our O-Shot procedure and seeing women with recurrent, hard-to-treat UTIs find relief from their symptoms.

Weโ€™ve discussed how the research suggests our procedure may be helpful for interstitial cystitis; however, all the papers except one focus on injecting into the bladder, and there has been limited discussion about treating recurrent UTIs.A book cover with a rocket launch Description automatically generated

But, many people have called me shocked that someone whoโ€™s suffered with horrible pain for a long time, diagnosed with interstitial cystitis, now has relief a week or so after an O-Shotยฎ for the first time in years.

And I have theories about why that might be happening: as you know, when we do the injection, weโ€™re literally millimeters from the urethra the way we do it. And sometimes, accidentally, we actually inject the urethra, so maybe thatโ€™s a good thing.

But definitely this antibacterial property of PRP may be helping with UTI, and of course the neovascularizationโ€”which would also help with fighting a chronic infectionโ€”could be part of why this happens.

Iโ€™m hesitating because I want to say this in a way that I donโ€™t over-promise or sound like Iโ€™m hawking some magic potion that fixes everything. But this is something that should be talked about more, because when we look at our naysayersโ€”the people that have never studied our procedureโ€”physicians donโ€™t really know what weโ€™re able to do and are closed-minded to the idea.

Some of our naysayers often list infection as a possible complication of PRP injections. Itโ€™s like saying youโ€™re going to cause asphyxia by having someone breathe oxygen. We donโ€™t cause infections. Weโ€™re treating with an antibiotic.

This is something I’ve woven into the email Iโ€™m going to give you.

Technique, Sterility, and the Importance of Proper PRP Preparation

This one has to do with technique.

One of the things I frequently preach about is the importance of having an FDA-cleared device for preparing platelet-rich plasma. And part of that has to do with the risk of contamination.

But (as opposed to using a tube meant to do lab testing), when you open up a RegenKit or a PureSpin or a Selphyl kit, theyโ€™re blister-packed. You could literally take them into the OR, and a circulating nurse could open them up and let the tube fall onto the sterile field.

Now, we donโ€™t maintain a sterile field during our procedures. Itโ€™s like the dentist doesnโ€™t maintain a sterile fieldโ€”heโ€™s all up in your mouth. You canโ€™t make it sterile. You can be clean. And so we do that, but weโ€™re not doing surgery. Weโ€™re administering an injection of platelet-rich plasma, a substance with antibacterial properties.

But on the other hand, when you are doing these proceduresโ€”when youโ€™re doing your phlebotomy and when youโ€™re dealing with the tubesโ€”you could technically, theoretically introduce contamination. And so they talk about risk of microbacterial, microbiome contamination when youโ€™re preparing, and they extend it to the whole environment.

So my recommendation has always just been as sterile as you would if you were starting an IV.

And, as you know, the face is so resistant to infection, and the genitalia, because theyโ€™re so vascular, there could be a tendency to maybe not be as diligent.

I mean, if you worked in the ER, you know people can fall out of their car and scrape their face on the gravel, get cut with a beer bottle, and then be dragged through the dirt in a fight on the way to the ERโ€”and they still hardly ever get an infection of the face; thereโ€™s so much vascularity.

And similarly with the genitaliaโ€”unless youโ€™re, of course, around the rectumโ€”but with just an injection or the genitalia, your chances of causing infection are minimal to none.

Improper Technique, Unauthorized Imitation, and Legal Consequences

On the other hand, itโ€™s worth noting that the wrong technique, the wrong kit, could lead to problems. And I think sometimes we take for granted the fact that we areโ€”because itโ€™s so routine for usโ€”we take for granted thatย people donโ€™t all know about what we do (they assume they know but often get it wrong).

Or the people who are copying usโ€”it happened in a nail salon, no doctor thereโ€”and they decide theyโ€™re going to do this on the sly out of a hotel room with this centrifuge they ordered off of Amazon. Those are the people who wind up in jail.

I know of two of them so far. Right after we started our Vampire Facelift, there was a massage therapist in California who went to prison. The initial news mentioned the Vampire Facelift. Turns out she had injected the buttocks with something she bought at the hardware store, but that patient just died.

But the point is that hereโ€™s a person whoโ€™s doing horrible things, claiming to be doing our procedure, and she was able to pull that off before we were able to shut her down.

And then the same thing happened a few years ago, as you know, in New Mexico, and those two ladies are now in prison.

I think itโ€™s worth noting, although we often take it for granted, that every now and then, sending out a paper like this is beneficial. It lets people know that we are following proper procedures as part of our protocol. Itโ€™s something subtle, but important.

Neurogenesis, Schwann Cells, Trauma, and Aging

This one is another nerveโ€”I mean, how many of these have we done? This oneโ€™s an author-opinion sort of article, but I like this right here:

PRP can stimulate Schwann cell proliferation, induce neurotropic factor synthesis, and significantly increase Schwann cell migration in a dose-dependent manner.

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This is something that I think we should also talk more about, because if you ask about it, youโ€™ll find quite a number of men and women who have noticed a decrease in sensation of the genitalia.

And I think some of itโ€™s bicycle injuries without people knowing it. I quit doing triathlons because I would get anesthesia of my genitals after a long ride and could never make that stop, even with the best of seats and bicycle shorts. And so it would be decreased sensation for ten minutes after I got off the bike.

Research supports that idea, but other trauma happens, right?[5]ย [6]ย [7]

A ten-pound baby passes through the birth canal and thereโ€™s physical trauma.

Even without trauma, there is a need for neurogenesis. DeLancey published studies where he did autopsies on aging females and documented very vast attenuation in the number of nerves in the clitoris and around the urethra.[8]

Thereโ€™s aging that triggers atrophy of muscle of the urinary sphincter,[9]ย and the same thing happens with nerve. And so even without injury, thereโ€™s a reason for promoting neurogenesis just because of age.

And we have a way of doing that. To me, thatโ€™s just tremendous. We can both grow nerves and blood flow, and weโ€™re doing it with what the body makes.

Exosomes, Stem Cells, and Regulatory Risk

I had another text messageโ€”someone asking me about exosomesโ€”and just be careful.

If you have any one for any reason who is saying to me, โ€œGo for it,โ€ (the devil or the angel on your shoulder), I always like to think, okay, if I goโ€”if I take their word on thisโ€”and then my worst outcome happens and the FDA shows up in my office, will there be a downside for this salesperson?

And the answer is none at all, unless youโ€™ve asked them to put in your hands what youโ€™re going to show to the FDA if they land in your office. If they cannot do that do not use the product, and if they do that and lied about it, okay, now theyโ€™re in trouble.

But Iโ€™ve seen it and Iโ€™ve heard about it among our group. These salespeople disappear. They change to another company, they go to the moon, and youโ€™re in trouble with the FDA or your medical board, and for the salesperson, there is no downside.

If they say you can use these exosomes by injection but you canโ€™t advertise it on your website, I would be very, very careful. Iโ€™m not going to tell you what to do, but I would be very, very careful. I like doing things I can talk about.

And I bring that up because thatโ€™s not going to happen with platelet-rich plasma. FDA doesnโ€™t care, and you donโ€™t have to have the exosomes to see our procedures work.[10]

Hereโ€™s a paper right here showing that our straight-up, old-school O-Shotยฎ worksโ€”which has become old school now that weโ€™re pushing into the second half of the second decade. I like saying it that way. Sixteen years makes the second half of the second decade.

But why go to something that puts you at risk legallyโ€”even if itโ€™s a small riskโ€”puts the patient at riskโ€”even if itโ€™s smallโ€”because it didnโ€™t come from their body, unless you need it?

Now, if you need itโ€”they didnโ€™t get well with your PRP, and you want to think about itโ€”then okay. But I think going straight to it, exosomes or stem cells, unless youโ€™re under an institutional review board or that salesperson puts something in your hand to make it safe for you if you get a knock on the door from their medical board or the FDA, then I would be very careful.

But all that to say, you canโ€”and I didโ€”weave this into an email that you can send to your patients, talking about how the science continues to support what we do. ย This paper is tremendously beautiful.

Review Articles, Visual Education, and Adjunctive Therapies

This one I put because itโ€™s justโ€”I admit itโ€”I like pretty pictures, and I like when someone goes to the trouble to do a really beautiful review article that clearly articulates a topic.[11]

So if youโ€™re treating androgenic alopecia, then this is a really nice article. You could literally print off and put it into your waiting room.

But the other option could be that you link to it and send it out along with an email, which is what I did for you. I wrote one for you.

My wife is doing this now, using intradermal botulinum toxin for alopecia, and others in our group.

This one againโ€”where did the exosomes come from? Are they from people or plants or the UPS man?

Just be careful. A lot of the studies, if you notice, where theyโ€™re more open about talking about it, are coming from other countries where they donโ€™t have an FDA.

And then this one I put in just becauseโ€”I mean, we know it worksโ€”but the saline arm, you know thatโ€™s my pet peeve to talk about: saline is not a placebo in a cellular therapy treatment with hydrodissection as a component.[12]ย [13]ย [14]

Saline worksโ€”decreased pain by 30%. PRP, of course, worked better for tendonitis.

Implementation Packet and Patient Email Strategy

Iโ€™ll open this up so you can read it. I have placed it in the handout section. I wrote two emails, and Iโ€™ll read through them for you, let you hear my thoughts, and offer some tips on how to send them out.

We lost a member today. I mean, we actually lost one to death last week. Our groupโ€™s big enough now that people retire and die, but we lost one who dropped out today.

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And he has a menโ€™s erection clinic that he operates on his own. Heโ€™s not part of a franchise or a big group. He just operates on his own.

Heโ€™d been in the group for a couple of months, and I looked. There was no webpage about the P-Shotยฎ. He never ordered any books or brochures or a poster to go into his office. And there was no form on his website to collect email addresses from people who might be interested in coming to see him.

=>Marketing Kit<= (members only)

This is something I hesitateโ€”I donโ€™t like sayingโ€”but itโ€™s true. Even if youโ€™re on our directoryโ€”and I know this is true because patients have told me and theyโ€™ve told our providersโ€”evenย if youโ€™re on our directory, if someone looks at your website and they canโ€™t find anywhere where youโ€™re talking about the procedure, even if youโ€™re in their town, they will get on an airplane and fly to another town to have the procedure.

Think about it.

You can buy a first-class ticket to anywhere in the U.S. for about a thousand bucks. If you want to fly coach, you can go round trip to most places in the US for 500 bucks or so.

Theyโ€™re spending two grand on a procedure. If they have the time and itโ€™s their genitals, they will go where they have connected with the provider online or in person.

And what I hear from most of our doctors (who are shocked when people fly from other places to see them) is the following: Theyโ€™ll ask the person, โ€œI see you came from such-and-such town where there resides a P-Shotยฎ provider near your home. Why did you fly to see me?โ€

Almost as if theyโ€™re scripted, theyโ€™ll say, โ€œI watched your video on your website. I watched your video.โ€

They want to see that the doctor who is going to treat them is all in.

Directory Upgrades and Member Visibility

I did want to show you something else before I get to the Implementation Kit. I keep forgetting to tell you guys this, but Iโ€™m really proud of this.

We upgraded our directories. It took a few months, but I want to show you what they do now.

A lot of people in the group think thatโ€™s the only reason for being in the group.

There are other reasons. For example, weโ€™ve had people who are making so much money, a colleague complained to the medical board.

And then I had to write a letter that said, โ€œLook, this person does what we do. Hereโ€™s 20 people that are professors of gynecology at various universities who do the same thing. Hereโ€™s a hundred papers to read. This is a real protocol, a real deal.โ€

And then it goes away.

Thatโ€™s happened seven or eight times now over the past 15 years.

Or someoneโ€™s next door and theyโ€™re advertising O-Shots for $300 and they are not licenses to use the name, maybe not even a licensed provider.

We pay for the attorneys to make that go away, and other things.

But the directory is helpful because your patients will look to see if youโ€™re on it, but it wonโ€™t do as much for you if you donโ€™t at least have a link to a page that you have.

But it will do more now than it used to.

So let me show you how itโ€™s workingโ€”one second.

Directory Functionality, Profiles, and Conversion Mechanics

Okay, Iโ€™ll useย the O-Shotยฎ directoryย as an example.

But look at this. So now what happens is we have some popular cities, and we have people in all these states.

If you just wanted to go straight to your state, you can click that. But the search option nowโ€”you can always click there and itโ€™ll just find out where you are and then find people close to you. And you can go by zip code, and you can go by countries, or you can go by states.

But itโ€™s the other thing that happensโ€”let me show you this and then Iโ€™ll quit talking about itโ€”but itโ€™s working a lot faster than it did.

Notice the difference:ย you can add your bio to the directory.

And that option was not there until aboutโ€”we started working on it several months ago, and we tightened it up so everything shows up prettier. Thatโ€™s probably not a real word, but more prettyโ€”but itโ€™s prettier, and thereโ€™s more functionality, and itโ€™s faster.

I also moved all the websites to a faster server, which a couple of weeks of my time.

So after you log in, if you look up in the right-hand corner, itโ€™ll say your name and itโ€™ll say โ€œEdit Profile.โ€ And if you click on โ€œEdit Profile,โ€ you just change it. You can change anything anytime you want. You donโ€™t have to call us.

Email Psychology, Implementation, and Positioning

Okay, let me show you the emails. You can download them, but Iโ€™ll show you the science and the psychology behind them, and then weโ€™ll call it a night.

Got two minutes to make it by 30 minutes.

Okay. Iโ€™m calling it my implementation packet, and Iโ€™m committed to bringing you guys one of these every week so you can just run with it.

And remember, my suggestion is that you personalize this, because it could belong to anybody. You personalize it by at least adding your picture. Of course, youโ€™re going to change the contact info, change it to a different closing.

I usually say โ€œSincerely.โ€ Some people have their little thing they say, like Roy Rogersโ€”what do you say? โ€œHappy Trails,โ€ or something.

I used to say โ€œPeace and Health,โ€ but have a closing so you donโ€™t spend time wasting on trying to think how you want to close that out.

Okay. So this oneโ€”look at the psychology of this.

โ€œDear First Name.โ€

If youโ€™ve been struggling with these problemsโ€”and this is written so the spam filters wonโ€™t grab it, because they willโ€”

โ€œThree recent scientific studies that we just talked about show that PRP helps damaged nerves.โ€

And then, if you want to turn that into an active link, you highlight it, copy it, and then, in the email software you use, look for the little chain link icon.

And then you just paste it.

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Iโ€™m not sure if youโ€™re able to see all that, but thatโ€™s an active link right there. See, I just made it active. So if I click on that, itโ€™ll take me there.

So you highlight it, and if you canโ€™t figure it out, do my Five Notes course, and Iโ€™ll show you how to do it.

Okay. This is one of the reasons PRP-based treatments such as our O-Shotโ€”I would change that to โ€œmyโ€ or โ€œourโ€ O-Shotโ€”can help improve, okay, natural antibacterialโ€”this is all true.

We have so manyโ€”I forgot about this when I wrote thisโ€”but we have people talking about vestibulitis and, of course, inflammatory conditions like lichen sclerosus, but also those recurrent UTIs.

And then here I wanted to brag about the fact that we know what weโ€™re doing. We have a protocol.

And so you get to knowโ€”just having a protocol, if you donโ€™t brag about it, why would you sell Tylenol and just have the label say โ€œacetaminophenโ€?

You are part of our group. So use the millionsโ€”literallyโ€”of money invested in our marketing and our reputation. And you have the right to do it.

So just put that in.

It helps sometimes to add the word โ€œprocedureโ€ to emphasize the fact that itโ€™s not just a shot. โ€œTreatmentโ€ is a good word. โ€œTreatments,โ€ โ€œprocedures.โ€ You see how Iโ€™ll put that in so that they know itโ€™s not just a simple shot.

Counterpoint: Cosmetic Gynecology Criticism and Evidence-Based Response

This oneโ€”I didnโ€™t cover this one. Let me pop back over and show you that.

They were giving cosmetic gynecologists a hard time.

And remember always pay attention to your enemies, because they tell you what shield to make.

If you say to me, โ€œYouโ€™re doing A, B, and C wrong,โ€ great.

Now that I read that, I know I need to either correct my error, or (if the criticism is unfounded) to shield against A, B, and C, which means I can talk about itโ€”the counterpoint to that.

I donโ€™t want to waste time trying to convince a closed mind, but if theyโ€™re out saying thatโ€”in other words, if they say, well, weโ€™ll use this as an exampleโ€”taking advantage of women by making them feel insecure about their labia so that we can make money off of them by doing surgical procedures to their labia.

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Okay? Thatโ€™s one of the cases they try to make in this paper.[15]

And we had the same thing happen when I first rolled out the O-Shotโ€”it was that I was taking advantage of women.

Well, no, weโ€™re not. Because 40% of women are psychologically bothered by their sexual dysfunction, and their families are strained. And research even shows their children are not as happy because of their sexual dysfunction.

So noโ€”sheโ€™s got a problem, and Iโ€™m trying to think of a way to help her.

And the same thing with this.

And so the paper I linked to is where they criticize us. And then I show the point and the counterpoint.

So let me swap back over because I forgot to show you that paper, and then weโ€™ll call it a night.

Sexual Dysfunction Prevalence, Distress Criteria, and AI Demonstration

Dr. Goodmanโ€”whoโ€™s in our group, Michael Goodmanโ€”is a legend. He was not only a pioneer in labioplasty, but a pioneer in endoscopic surgery. Brilliant man.

He published this study and others and showed that when women have labioplastyโ€”even if itโ€™s just for cosmetic reasonsโ€”their sexual function improves.[16]ย [17]ย [18]

So if weโ€™re helping them in the bedroom, then weโ€™re not taking advantage of them.

Now, most of us are not surgeons. Many in our group are. Most of us are not labioplasty surgeons.

But the other thing thatโ€™s happening when I go to their conferencesโ€”at least I would say 90-plus percent of the lectures by โ€œcosmeticโ€ gynecologists is regarding dysfunction (not beauty alone).

Itโ€™s almost an unfortunate name (aesthetic/cosmetic gynecology), because even though itโ€™s called cosmetic gynecology, theyโ€™re dealing with things like phimosis secondary to lichen sclerosus, where you canโ€™t even retract the clitoral hood.

And often, the woman has seen two or three gynecologists who never even noticed. Itโ€™s tragic.

Many of us have seen that repeatedly.

And of course, the husband doesnโ€™t know, and the wife can hardly see down there.

And all doctorsโ€”weโ€™re trained to examine the glans penis by retracting the foreskin. And I think there are 16,000 or so men who get squamous carcinoma of the penis every year and we know how to look for it.

It’s a horrible thing to have your penis cut off.

And we were taught to check the glans penis and teach men how to retract their foreskin and keep the area clean. But the analogous instruction by physicians (retracting the clitoral hood to clean the area) is still not happening with women.

Remember that paper we talked about a lot? Only one in seven medical schools was teaching gynecologists the full clitoral anatomy.[19]

And so itโ€™s no wonder that we canโ€™t hold the husband responsible for not knowing the anatomy if the gynecologist doesnโ€™t likely know the anatomy of the clitoris.

Not stupid peopleโ€”just an inadequate education system thatย we as a groupย are trying to change.

Q&A: Prevalence of Female Sexual Dysfunction

Question: Is there a reference or source where I can reference and read about the 40% of sexual dysfunction?

โ€œCan you give me more references about the percentages of women who suffer with sexual dysfunction?โ€

Thereโ€™s a lot of discussion about what the actual number is, but conservatively, itโ€™s 30 to 40%.

And the other trick isโ€”to be counted, you have to be psychologically bothered by it.

You canโ€™t just say, โ€œOh, I have dyspareunia, but I donโ€™t care because I donโ€™t have sex.โ€

If you have that, you do not have sexual dysfunction.

Among 18 studies, female sexual dysfunction ranged from 20 to 95%. The pooled estimate was 47%.

Across 135 studies in 41 countries, 41% of reproductive-age women were affected.[20]ย [21]ย [22]ย [23]ย [24]

And yet you have one freaking medical school out of seven that teaches gynecologists how to do a history and physical on a woman with sexual dysfunction.

Itโ€™s a tragedy of medicine.

Okay. Sorryโ€”Iโ€™m ranting. Better call it a night.

Thank you for being on the call.

Bye-bye.

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References

Abdelgeliel, Asmaa Sayed, Waiel F. Sayed, Wesam M. Salem, and Fatma S. Hassan. โ€œAntibacterial and Antibiofilm Activity of Platelet-Rich Plasma under Different Activation Conditions against Multidrug-Resistant MRSA Isolated from Human Skin Abscesses.โ€ย BMC Biotechnology, ahead of print, December 8, 2025. https://doi.org/10.1186/s12896-025-01078-x.

Adebisi, Omoniyi Y., and Karen Carlson. โ€œFemale Sexual Interest and Arousal Disorder.โ€ Inย StatPearls. StatPearls Publishing, 2025. http://www.ncbi.nlm.nih.gov/books/NBK603746/.

Aggour, Reham L., and Lina Gamil. โ€œAntimicrobial Effects of Platelet-Rich Plasma against Selected Oral and Periodontal Pathogens.โ€ย Polish Journal of Microbiologyย 66, no. 1 (2017): 31โ€“37. https://doi.org/10.5604/17331331.1235227.

Asghar, Aneela, Zahid Tahir, Aisha Ghias, Usma Iftikhar, and Tahir Jameel Ahmad. โ€œEfficacy and Safety of Intralesional Normal Saline in Atrophic Acne Scars.โ€ย Annals of King Edward Medical Universityย 25, no. 2 (2019): 2. https://doi.org/10.21649/akemu.v25i2.2867.

Bagherani, Nooshin, and Bruce R Smoller. โ€œIntroduction of a Novel Therapeutic Option for Atrophic Acne Scars: Saline Injection Therapy.โ€ย Global Dermatologyย 2, no. 6 (2016). https://doi.org/10.15761/GOD.1000159.

Beitzel, Knut, Donald Allen, John Apostolakos, et al. โ€œUS Definitions, Current Use, and FDA Stance on Use of Platelet-Rich Plasma in Sports Medicine.โ€ย The Journal of Knee Surgeryย 28, no. 1 (2015): 29โ€“34. https://doi.org/10.1055/s-0034-1390030.

Cl, Karan, Madhan Jeyaraman, Naveen Jeyaraman, Swaminathan Ramasubramanian, Manish Khanna, and Sankalp Yadav. โ€œAntimicrobial Effects of Platelet-Rich Plasma and Platelet-Rich Fibrin: A Scoping Review.โ€ย Cureus, ahead of print, December 30, 2023. https://doi.org/10.7759/cureus.51360.

Codispoti, Nicolette, Olivia Negris, Monica C Myers, et al. โ€œFemale Sexual Medicine: An Assessment of Medical School Curricula in a Major United States City.โ€ย Sexual Medicineย 11, no. 4 (2023): qfad051. https://doi.org/10.1093/sexmed/qfad051.

El-Amawy, Heba Saed, and Sameh Magdy Sarsik. โ€œSaline in Dermatology: A Literature Review.โ€ย Journal of Cosmetic Dermatologyย 20, no. 7 (2021): 2040โ€“51. https://doi.org/10.1111/jocd.13813.

Goodman, Michael, Samantha Fashler, John R Miklos, Robert D Moore, and Lori A Brotto.ย The Sexual, Psychological, and Body Image Health of Women Undergoing Elective Vulvovaginal Plastic/ Cosmetic Procedures: A Pilot Study. 28, no. 4 (2011): 9.

Goodman, Michael P., Otto J. Placik, Royal H. Benson, et al. โ€œA Large Multicenter Outcome Study of Female Genital Plastic Surgery.โ€ย The Journal of Sexual Medicineย 7, no. 4 (2010): 1565โ€“77. https://doi.org/10.1111/j.1743-6109.2009.01573.x.

Goodman, Michael P., Otto J. Placik, David L. Matlock, et al. โ€œEvaluation of Body Image and Sexual Satisfaction in Women Undergoing Female Genital Plastic/Cosmetic Surgery.โ€ย Aesthetic Surgery Journalย 36, no. 9 (2016): 1048โ€“57. https://doi.org/10.1093/asj/sjw061.

Jaafarpour, Molouk, Ali Khani, Javaher Khajavikhan, and Zeinab Suhrabi. โ€œFemale Sexual Dysfunction: Prevalence and Risk Factors.โ€ย Journal of Clinical and Diagnostic Researchโ€ฏ: JCDRย 7, no. 12 (2013): 2877โ€“80. https://doi.org/10.7860/JCDR/2013/6813.3822.

Khani, Ali. โ€œFemale Sexual Dysfunction: Prevalence and Risk Factors.โ€ย JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, ahead of print, 2013. https://doi.org/10.7860/JCDR/2013/6813.3822.

Litwinowicz, Kamil, Marcin Choroszy, and Anna Wrรณbel. โ€œStrategies for Reducing the Impact of Cycling on the Perineum in Healthy Males: Systematic Review and Meta-Analysis.โ€ย Sports Medicine (Auckland, N.z.)ย 51, no. 2 (2021): 275โ€“87. https://doi.org/10.1007/s40279-020-01363-z.

Lui, Hansen, Nnenaya Mmonu, Mohannad A. Awad, et al. โ€œAssociation of Bicycle-Related Genital Numbness and Female Sexual Dysfunction: Results From a Large, Multinational, Cross-Sectional Study.โ€ย Sexual Medicineย 9, no. 3 (2021): 100365. https://doi.org/10.1016/j.esxm.2021.100365.

Mitchell, Kirstin R., Kyle G. Jones, Kaye Wellings, et al. โ€œEstimating the Prevalence of Sexual Function Problems: The Impact of Morbidity Criteria.โ€ย Journal of Sex Researchย 53, no. 8 (2016): 955โ€“67. https://doi.org/10.1080/00224499.2015.1089214.

Nappi, Rossella E., Laura Cucinella, Silvia Martella, Margherita Rossi, Lara Tiranini, and Ellis Martini. โ€œFemale Sexual Dysfunction (FSD): Prevalence and Impact on Quality of Life (QoL).โ€ย Maturitasย 94 (December 2016): 87โ€“91. https://doi.org/10.1016/j.maturitas.2016.09.013.

Panara, Kush, John M. Masterson, Luis F. Savio, and Ranjith Ramasamy. โ€œAdverse Effects of Common Sports and Recreational Activities on Male Reproduction.โ€ย European Urology Focusย 5, no. 6 (2019): 1146โ€“51. https://doi.org/10.1016/j.euf.2018.04.013.

Pandit, Meghana, John O L Delancey, James A Ashton, Jyothsna Iyengar, Mila Blaivas, and Daniele Perucchini.ย Quantification of Intramuscular Nerves Within the Female Striated Urogenital Sphincter Muscle. 2005.

Pipitone, Fernanda, Zhina Sadeghi, and John O. L. DeLancey. โ€œUrethral Function and Failure: A Review of Current Knowledge of Urethral Closure Mechanisms, How They Vary, and How They Are Affected by Life Events.โ€ย Neurourology and Urodynamicsย 40, no. 8 (2021): 1869โ€“79. https://doi.org/10.1002/nau.24760.

Sethi, Dalip, Kimberly E. Martin, Sangeeta Shrotriya, and Bethany L. Brown. โ€œSystematic Literature Review Evaluating Evidence and Mechanisms of Action for Platelet-Rich Plasma as an Antibacterial Agent.โ€ย Journal of Cardiothoracic Surgeryย 16, no. 1 (2021): 277. https://doi.org/10.1186/s13019-021-01652-2.

Shin, Jung-Won, and Chang-Hun Huh. โ€œUpdates in Treatment for Androgenetic Alopecia.โ€ย Annals of Dermatologyย 37, no. 6 (2025): 327โ€“35. https://doi.org/10.5021/ad.25.042.

Syed, Shamail A, and Asila Anwar. โ€œThe Globally Rising Tide of Cosmetic Gynaecology: Are Providers Aware of the Ethical Aspects?โ€ย Cureus, ahead of print, November 7, 2025. https://doi.org/10.7759/cureus.96327.

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[1]ย Abdelgeliel et al., โ€œAntibacterial and Antibiofilm Activity of Platelet-Rich Plasma under Different Activation Conditions against Multidrug-Resistant MRSA Isolated from Human Skin Abscesses.โ€

[2]ย Aggour and Gamil, โ€œAntimicrobial Effects of Platelet-Rich Plasma against Selected Oral and Periodontal Pathogens.โ€

[3]ย Cl et al., โ€œAntimicrobial Effects of Platelet-Rich Plasma and Platelet-Rich Fibrin.โ€

[4]ย Sethi et al., โ€œSystematic Literature Review Evaluating Evidence and Mechanisms of Action for Platelet-Rich Plasma as an Antibacterial Agent.โ€

[5]ย Panara et al., โ€œAdverse Effects of Common Sports and Recreational Activities on Male Reproduction.โ€

[6]ย Lui et al., โ€œAssociation of Bicycle-Related Genital Numbness and Female Sexual Dysfunction.โ€

[7]ย Litwinowicz et al., โ€œStrategies for Reducing the Impact of Cycling on the Perineum in Healthy Males.โ€

[8]ย Pandit et al.,ย Quantification of Intramuscular Nerves Within the Female Striated Urogenital Sphincter Muscle.

[9]ย Pipitone et al., โ€œUrethral Function and Failure.โ€

[10]ย Beitzel et al., โ€œUS Definitions, Current Use, and FDA Stance on Use of Platelet-Rich Plasma in Sports Medicine.โ€

[11]ย Shin and Huh, โ€œUpdates in Treatment for Androgenetic Alopecia.โ€

[12]ย Asghar et al., โ€œEfficacy and Safety of Intralesional Normal Saline in Atrophic Acne Scars.โ€

[13]ย Bagherani and R Smoller, โ€œIntroduction of a Novel Therapeutic Option for Atrophic Acne Scars.โ€

[14]ย El-Amawy and Sarsik, โ€œSaline in Dermatology.โ€

[15]ย Syed and Anwar, โ€œThe Globally Rising Tide of Cosmetic Gynaecology.โ€

[16]ย Goodman et al., โ€œA Large Multicenter Outcome Study of Female Genital Plastic Surgery.โ€

[17]ย Goodman et al., โ€œEvaluation of Body Image and Sexual Satisfaction in Women Undergoing Female Genital Plastic/Cosmetic Surgery.โ€

[18]ย Goodman et al.,ย The Sexual, Psychological, and Body Image Health of Women Undergoing Elective Vulvovaginal Plastic/ Cosmetic Procedures: A Pilot Study.

[19]ย Codispoti et al., โ€œFemale Sexual Medicine.โ€

[20]ย Mitchell et al., โ€œEstimating the Prevalence of Sexual Function Problems.โ€

[21]ย Nappi et al., โ€œFemale Sexual Dysfunction (FSD).โ€

[22]ย Jaafarpour et al., โ€œFemale Sexual Dysfunction.โ€

[23]ย Khani, โ€œFemale Sexual Dysfunction.โ€

[24]ย Adebisi and Carlson, โ€œFemale Sexual Interest and Arousal Disorder.โ€


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